›› 2012, Vol. 24 ›› Issue (3): 166-171.doi: 10.3969/j.issn.1004-616x.2012.03.002

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The effect of lead and cadmium on mRNA of S100 calcium-binding protein family members in JAR cells

ZHU Min，ZHANG Qing-ying，LUO Jia-yi，ZHANG Dong-hong，ZHAO Zhi-guo，YI De-qing   

  1. 1. Department of Preventive Medicine, Shantou University Medical College, Shantou 515041; 2. Cardiovascular Research Center, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2011-12-20 Revised:2012-02-29 Online:2012-05-30 Published:2012-05-30
  • Contact: ZHANG Qing-ying

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Abstract: OBJECTIVE: To explore the effects of lead acetate (Pb) and cadmium acetate (Cd) on the mRNA expression of S100 calcium-binding protein family members in JAR cells. METHODS：MTT Viability Test was performed to determine the median lethal dose (LC50) in JAR cells. JAR cells were treated by different concentrations of PbAc and CdAc for 24 h and 48 h. Expressions of S100 proteins mRNA by RT-PCR between different experimental groups and control group was compared. RESULTS：After PbAc (2.5-20 μmol/L) and CdAc (2.5-10 μmol/L) treatmeat of JAR cells，the relative cell viability showed a negative correlation with concentration (r = -0.992 4 or -0.995 5，P < 0.05 for both). The median lethal concentration (LC50) of PbAc was 23.15 μmol/L，and that of CdAc was 8.30 μmol/L. Among the nine S100 calcium-binding protein family members，only S100A10， S100A11， S100A14 and S100P were found to be significantly expressed，while others showed lower expression or no expression by RT-PCR. The mRNA expression of S100A14 in JAR cells treated with PbAc and CdAc after 24 h and 48 h were significantly different from the negative control (P <0.05)，while no significant difference was found for the expression of S100A1 and S100A11. When JAR cells were treated with 2.5 μmol/L PbAc for 24 h，the expression of S100P significantly increased (P <0.05)，but expression declined as the dose of PbAc increased. When JAR cells were treated with CdAc for 24 h，the expression of S100P significantly increased (P <0.05). However after the exposure of PbAc and CdAc for 48 h，S100P expression of all experimental groups were increased，with no significant difference compared with the control group. CONCLUSION：PbAc and CdAc could increase the mRNA expressions of S100A14 and S100P in JAR cells，and the variation of S100P expression was similar to metallothionein (MT). The enhanced mRNA expressions of S100A14 and S100P may be involved in heavy metal toxic stimulation stress response. We postulate that they may protect cells from the toxic effects of heavy metals，and they might be molecular biomarkers for stress response.

Key words: lead acetate, cadmium acetate, JAR cells, S100 calcium-binding protein

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